Biology Stack Exchange
Biology Stack Exchange

Questions tagged [gene-expression]

The process by which information encoded in a gene is converted into a functional protein or RNA, resulting in or contributing to a phenotype.

The process by which information encoded in a gene is converted into a functional protein or RNA, resulting in or contributing to a phenotype.

371 questions
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Time from stimulus to gene expression

My understanding is that gene expression, in response to some stimulus, generally occurs on the order of minutes. I'm curious about the extremes...the quickest and the slowest cases. What is(are) the fastest time(s) recorded for genes being…
Daniel Standage
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How does a gene "know" what to change to?

Excuse my ignorance but I've always been curious about this... For example, a frog is red, but it starts living in a green forest. Over time the frog becomes green to camouflage. But a gene can't see and I'm sure there's no mechanism for color info…
user36637
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Which is more important for protein expression mRNA structure or codon optimization?

The field seems extremely divided on the debate. On one hand, artificial experiments have suggested that synonymous mutations don't correlate with gene expression but rather, the mRNA 5' structure is the most important 1. On the other hand, genome…
bobthejoe
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Determining potential protease sites within a recombinant protein

My expressed proteins are frequently truncated and I'm trying to figure out which bands are which. The first thing to come to mind is using PeptideCutter from ExPASy but there is just a data deluge of potential sites. I was curious what other…
bobthejoe
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Average number of gene products in (a) eukaryote(s)

Due to alternative RNA splicing, it isn't uncommon to ultimately find multiple gene products expressed from one gene in eukaryotes. I'm looking for a reference value for the average number of final gene products expressed per gene for: ... a…
LanceLafontaine
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What is meant by "alien" probe in a microarray?

In my lab we did a microarray to analyze differential gene expression in S. cerevisiae treated with UV irradiation. We are now analyzing the results and one of the up-regulated genes is labeled "Alien4_60." I believe this is some kind of control,…
RyanM
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Why are protein-coding regions rich in GC?

I have been searching for an answer for this question and have some possible solutions, but I am not sure. GC regions are more stable as there are 3 hydrogen bonds instead of 2 with AT, however I am not sure if this would influence the number of…
user48207
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What is the role of TATA box in transcriptional regulation?

I know what is the TATA box, but I wish to know whether it has specific roles in transcriptional regulation.
InfZero
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2 blue eyed children

My husband had light brown eyes. His father had hazel and his mother, light brown as well. His younger brother however, has blue eyes. Both my children have blue eyes. Is this possible? I thought once the recessive gene was used in my first child,…
user5715
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How do substances for gene expression work?

How does amplification of gene expression work? I often find in various articles expressions like "Using substance X or peptide X, we enhanced the expression of gene Z, which led to a certain therapeutic effect. How to find out which substance…
Дмитрий Смирнов
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qPCR: Huge variation in fold change of genes between biological replicates

I am trying to validate my RNAseq data by doing qpcr for which I am looking at the fold change of few genes across various timepoints of treatment conditions. I am getting huge amount of variation (in thousand folds ) in my biological replicates. I…
ambika
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Cell type that expresses 90% of the genome

Cells that are differentiated express the genes that are necessary for their own usage. I've heard that some cell type expresses about 90% of 30,000 proteins that are encoded in the genome. Can anybody tell me what this cell type is?
박성우
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What is the difference between differentially expressed genes and deregulated genes?

Can anyone explain clearly what is the difference between differentially expressed genes and deregulated genes?
stack_learner
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Is the increased/decreased enzyme activity (tyrosinase) caused by an environmental factor (UV radiation) considered to be gene expression?

My AP Bio assignment asks that I research the effects of UV radiation on melanin production, but the directions and questions suggest that UV radiation influences gene expression. From what I could find, such as on this university site or this…
Aleksandr Hovhannisyan
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Random X-Inactivation and Duchenne

I'm reading about X-inactivation and I can't reconcile some things with it being truly random. In only a small percentage of female carriers Duchenne's will be expressed. But if this was truly random, wouldn't 50% of female carriers expressing the…
Rommelaar
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