I recommend a dual approach in these situations. Indicate that based on your experience/degree/and market value you believe that 80k is the appropriate salary that your skills deserve, however, also indicate that you want the best company even if it's not quite at that level and that for the right company you would negotiate in order to get a better work environment.
This puts most everything into the discussion of the interview where you can talk to them and find the "best" fit with them. If they know what you are looking for in a company and they fit it 100% then they will likely still extend an offer to you after you assure them the environment is a priority and that theirs is the best, if they think your skills are worth it, then likely they will come in closer than the bottom of their tier. If however, it's not a fit then you will both know that during the interview and they will likely not bother extending, but shoot for someone more in their price range.
The key is to focus on the company quality and work environment and match that up with your expectations (benefits included) and that naturally everyone wants more base pay, but that the environment is so very important to you. Get them to sell their work culture, environments, benefits, and not just focus on the bottom line. If it's literally just the bottom line that is the sole concern then don't bother unless you are willing to take a companies range. Then when you go in hit the top of their range and work negotiations from there. Otherwise request the amount you want and let it go how it goes.