Connection Timeout Expired. The timeout period elapsed while attempting to consume the pre-login handshake acknowledgement. This could be because the pre-login handshake failed or the server was unable to respond back in time.
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It seems to be a firewall issue. Firewall seems to be blocking connection to sql server for port 1433. You will need to add a rule to allow connection on this port.
You can find more on this here: SQL Server Pre-Login Handshake Acknowledgement Error
Sujith
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After 2 Months R &D on Web Server And SQL SERVER I Got Solution For The Connection pool Error I Have Found the Solution for Connection Pooling in ASP.NET and MVC as WELL, Set the WEB SERVER Settings Properly Go The Web Server Select the Connection Pool , select the Application Pool then , Select Which site You are facing, Then Right Click on The Pool Name -->Select Adv Settings Set the Max Worker Processors "1" to 6000 .Then You never Face This Problem. in my Case Set Max Worker Processors to 6000 – KASHI Jul 11 '17 at 12:46