Promoter from the bateriophage T7. The promoter is highly specific for the T7-RNA polymerase and widely used in the field of molecular biology as promoter for controlled gene expression.
Questions tagged [t7-promoter]
11 questions
10
votes
4 answers
What determines a successful protein expression in E. coli?
Some proteins express well in a heterologous host; others- don't. A few requirements are known to determine the protein expression, like a strong promoter (like T7) for transcription and a strong ribosome binding site for translation. I am working…
Gergana Vandova
- 6,925
- 2
- 36
- 65