A predetermined methodology for carrying out an experiment.
Questions tagged [protocol]
84 questions
13
votes
1 answer
How straightforward is in vitro compartmentalization?
in vitro compartmentalization (IVC) is one of those technologies that everyone knows about, talks about, but never actual does due to the rather technical difficulties in setting the system up. I was wonder if anyone not named Tawfik, Griffiths or…
bobthejoe
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5
votes
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How to set up a slow cooling on an AB Veriti thermal cycler?
I want to incubate my sample at 50ºC and then slowly cool it down to 4ºC at a rate of 0.1ºC/s. I am using AB Veriti thermal cycler. Does anyone know how to set up the rate of cooling? It has to do something with the ramp rate, which is given in %…
Gergana Vandova
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2
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0 answers
Difference between cytospin and metaphase spread protocol
I would like to know what exactly is the difference between cytospin and metaphase protocol. I'm a data scientist helping a medical lab and I receive images of cells with particular types of DNA. The DNA comes stained with DAPI. However, other…
user37831
1
vote
0 answers
How to troubleshoot islet isolation
I have been getting very low yield from islet isolations from rats.
My procedure is as follows:
Inject the pancreas with 5mL Liberase (1 wunch unit/ml) in HBSS + HEPES through the CBD (decent inflation, atleast of the frontal part of the pancreas)…
Jesper Madsen
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1 answer
reason for "not for diagnostic use" advice in protocols
I read "not for diagnostic use" in every protocol i saw. why is it so?
which differences have the kits and material that are used for diagnostic purposes?
Katz
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