I have some mouse tumours that have been snap frozen in liquid nitrogen following an in vivo study, and I thought it might be interesting to also determine the weight of the tumours. I was wondering if weighing frozen tumours would still be accurate, or if I should have measured them before they were frozen? Would one expect the weight to be significantly altered just by snap freezing samples?
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If the tumours were sealed before being frozen, then of course the weight is the same. The temperature of something does not change its mass. However, the frozen ones might be heavier if, during the process, more water (or other substances) got trapped into the tumour. At the end this is a problem of enginneering and about your freezing method and not a question of biology. – Remi.b May 27 '19 at 14:11
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I'm voting to close this question as off-topic because it is a question of engineering of the freezing technique and not a question of biology. – Remi.b May 27 '19 at 14:11
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1I think this is specific enough to a biological technique that it's on topic. – Bryan Krause May 27 '19 at 23:00
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1I agree with Bryan; closing this question would be failing to see the forest for the trees. – Michael_A May 28 '19 at 00:07